FUMIGATION OF MICROBIOLOGY LAB

1.0 OBJECTIVE
To lay down a procedure for Fumigation of Microbiology Lab.
2.0 SCOPE
This SOP is applicable to Quality Control Department (Microbiology Lab).
3.0 RESPONSIBILITY
Microbiologist
4.0 ACCOUNTABILITY
Department Head.
5.0 PROCEDURE
5.1 Use safety goggles and hand gloves during fumigation.
5.2 Put ‘OFF’ the A/C unit .
5.3 Take Potassium Permanganate (about 15 gm.) in a petri dish and keep in the area where fumigation is to be carried out.
5.4 Place the petri plate on a polyethene bag.
5.5 Add about 25 ml of 35 % Formaldehyde solution to Potassium Permanganate .
5.6 Immediately close the area.
5.7 Label the area as “Area Under Fumigation” so that nobody enters the room.
5.8 Keep the room under fumigation for a minimum of 8 - 12 hrs.
5.9 Defumigate the room by putting on the A/C unit .
6.0 FREQUENCY :
6.1 once a week and after completion of any civil work.
Hazards of Formaldehyde :
1. It is toxic to inhalation and ingestion and causes irritation to eyes and respiratory tract.
2. Skin contact can cause dermatitis. Also cause skin burns.
3. Exposure to a concentration of 10 to 20 PPM can cause difficulty in breathing.
4. Slightly corrosive in nature, suspected carcinogen.
Fire Hazards :
1. Vapors of Formaldehyde are flammable and may form an explosive mixture with air.
2. It should be stored in a cool and well ventilated area.
3. Avoid storing below 16 Degree C because it forms a polymer ‘Paraformaldehyde’ which is a flammable solid which on heating may evolve a flammable gas and may explode.