IDENTIFICATION OF BACTERIAL ISOLATES

1.0 OBJECTIVE
To lay down the procedure for identification of the bacterial colonies obtained during the plate exposure in production area to know about the flora present in the production environment.
2.0 SCOPE
This SOP is applicable to the Quality Control Department (Microbiology Lab).
3.0 RESPONSIBILITY
Microbiologist/ Quality Control Executive
4.0 ACCOUNTABILITY
Department Head.
5.0 PROCEDURE
5.1 Pick the individual colonies from the plates exposed in change rooms, secondary packing rooms and finished goods stores.
5.2 Purify the colonies, if necessary by streaking on to Soyabean casein digest agar plate.
5.3 Observe and record the morphological characters of the colonies such as shape, elevation, colour and margins in . Record all the observation.
5.4 Using sterile inoculating technique, inoculate the organism on two soyabean casein digest agar slants. Use one slant culture to determine the cultural characteristics and the other to repeat any of the tests , if necessary as stock.
5.5 Make a smear of the colony by taking with a loop and subject it to Gram’s Staining procedure. Observe under microscope and note the reaction , morphology and arrangement of the cells.
5.6 Using aseptic technique ,inoculate the required media , to carry out the biochemical tests and incubate at 37°C for 24 to 72 hrs. Record the results.