PROCEDURE FOR PREPARATION OF CULTURE MEDIA

1.0 OBJECTIVE
To lay down a procedure for preparation of culture media.
2.0 SCOPE
This SOP covers the procedure for preperation of culture media and is applicable to Quality Control Department
3.0 RESPONSIBILITY
Microbiologist.
4.0 ACCOUNTABILITY
Department Head
5.0 PROCEDURE
5.1 Store dehydrated media in tightly closed bottles in the dark or as directed by manufacturer.
5.2 Follow the manufacturers direction for rehydration .
5.3 Check pH of the medium after rehydration and if necessary adjust the pH.
5.4 After rehydrating the medium dispense promptly into labeled flasks or tubes and cover with metal caps / plastic caps/ cotton plugs and wrap in butter paper and sterilize within two hours as per the manufacturers instructions.
5.5 Incubate the sterilized media for 24 hrs. before use at the appropriate temperature to check the contamination if any.
5.6 Store the media below 25 OC after pre-incubation and use within a week of preparation.
5.7 Check the nutritive properties of each new batch of medium using 100 cfu of the appropriate test organism and record the observation in format.
5.8 Use the dehydrated media as per the expiry given on container.
5.9 Records the preparation and sterilization of the culture media in a log book.
REASON FOR REVIEW
1. Routine review.
2. Scope modified.