MALAYSIA ACTD GUIDELINES

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CALIBRATION OF UV-VISIBLE SPECTROPHOTOMETER

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1.0 CONTROL OF ABSORBANCES PROCEDURE: REPARATION OF POTASSIUM DICROMATE SOLUTION: Dry K2Cr2O7 at 130oC for 2 hours. Weigh accurately about the quantity of sample equal to 60mg into 1000ml volumetric flask. Add 0.005m Sulphuric acid to dissolve and dilute with the same solvent. Find out the absorbances of (A 1% 1cm) above said solution at wavelengths of a 235nm, 257nm, 313nm and 350nm. Calculate the (1% 1cm) value using the following expression. Absorbance X 1 X 1000 / Weight of K2Cr2O7 in g X 100 Check the value for each wavelength with the tolerances given four wavelengths. PREPARATION OF REAGENT: (i) Dilute 3ml of concentrated sulphuric acid and make up 100ml with distilled water. (ii) Dilute 20ml to 2000ml with distilled water. 2. LIMIT OF STRAYLIGHT: The absorbance of a 1.2-% w/v solution of potassium chloride at a path-length of 1cm should be greater than 2.0 at about 200nm when compared with water as reference liquid. 1.2% POTASSIUM CHLORIDE SOLUTION: Take 1.2 g of Potassium chlo...
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Instrument Operation of Antibiotic Zone reader

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1.0 Purpose : The purpose of this procedure is to describe the method of operation of Antibiotic zone reader. 2.0 Scope : This procedure applicable to Antibiotic zone reader used in QC department (microbiology department) 3.0 Responsibility : Manager QC & Microbiologist QC. 4.0 Procedure : Check that the instrument is clean and kept in proper position. Connect the power cable to the mains and switch ‘ON’. Switch ‘ON’ the mains in the instrument. Ensure that the zero of the main scale coincides with zero of the main scale coincides with zero in the vernier scale. Place the petridish in such a way that the lower edge of the zone coincides with the mark line on the prism and in the centre of the magnifier. Rotate the left side knob anticlockwise till the upper edge of the zone coincides with the mark line on the prism. Note down the reading in mm with respect to the main scale and vernier. Rotate the stage anticlockwise using the handle provided at the left hand side and bring ...
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PROCEDURE FOR FUMIGATION OF CLEAN ROOMS

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1.0 Formaldehyde Solution 37% (Formalin) sufficient to vapourise about 35 ml of Formalin for each cubic metre of enclosed space. a) Large stainless steel evaporating pans. b) Thermostatically controlled hot plates. c) Small stainless steel canisters with perforated stainless steel lids. Polyurethane pieces. d) Adhesive tapes e) Goggles f) Aseptic area garments, duly sterilized g) Thermometers h) Petri dishes with nutrient agar media. 2.0 PRECAUTIONS 2.1 Formalin is a strong irritant and is toxic. Inhalation of formaldehyde gas should be avoided since it cause irritation of the respiratory tract. Contact with the skin should also be avoided. 2.2 When Formalin is being handled, protective clothing inclusive of rubber gloves and goggles must be worn. Personnel must leave the area being fumigated as soon as possible after placing the pans of Fornalin on the hot plate...
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