OPERATING PROCEDURE FOR DIANA AIR SAMPLER (MICROMED)

1.0 OBJECTIVE:
To estimate Microbial Load in Production Area by Air sampling kit.
2.0 SCOPE
This SOP is applicable to Quality Control Department (Microbiology Lab).
3.0 RESPONSIBILITY:
Sr. Executive.
4.0 ACCOUNTABILITY :
Department Head
5.0 PROCEDURE:
5.1 Take sampling kit to area where sampling to be carried out.
5.2 Remove the sampler from kit and open the backside lid .
5.3 Put batteries in the sampler and ensure that it is in working condition.
5.4 Put the sterilized hand gloves.
5.5 Remove the strip holder and sanitize the complete assembly by isopropyl alcohol
(70% filtered through 0.2u membrane filter).
5.6 Remove agar strip from sealed sterile pouch and insert carefully into the strip holder.
5.7 Adjust the timer by a knob provided and set the time from 0 to 1 minute , 2 minute
upto maximum 4 minutes.
5.8 Press starts button .
5.9 After completion of sampling remove the agar strip and again put carefully in the sterile pouch.
5.10 Incubate the strip in an incubator at 37 °C for five days.
5.11 Finally sanitize the air sampler thoroughly and keep it sampler box .
6.0 PRECAUTION
6.1 Do not autoclave the air sampler kit , electronic circuit will get spoiled.
6.2 Handle carefully and check the batteries for spillage if any.