CALIBRATION OF FLUORIMETER

1.0 PROCEDURE :
1.1 PREPARATION OF STANDARD STOCK SOLUTION OF QUININE SULFATE:
Weigh accurately 10mg of Quinine sulfate and dissolve in 0.1N Sulfuric acid and make up to volume with 0.1N Sulfuric acid to 1000mL. Preserve this solution, protected from light, in a refrigerator.
For calibration, dilute the above stock solution as follows:
Soln. 1.5ml - 200ml with 0.1 N Sulfuric acid (0.25 mcg/ml)
Soln. 2.4ml - 200ml with 0.1 N Sulfuric acid (0.20 mcg/ml)
Soln. 3.3ml - 200ml with 0.1 N Sulfuric acid (0.15 mcg/ml)
Soln. 4.2ml - 200ml with 0.1 N Sulfuric acid (0.10 mcg/ml)
Soln. 5.1ml - 200ml with 0.1 N Sulfuric acid (0.05 mcg/ml)
1) Select the instrument as per the Operating Procedure.
2) Select Primary filter (366 nm) and Secondary filter (4308 nm)
3) Adjust the blank to zero with 0.1 N Sulfuric acid.
4) Place the diluted solution 1 in the cuvette. Adjust the reading to 100 and place the other diluted solutions in the cuvette and note down the readings.