INSTRUMENT CALIBRATION PROCEDURE GAS CHROMATOGRAPHY

1.0 PURPOSE :
To provide a procedure for Calibration of Gas Chromatography
2.0 SCOPE:
This covers the procedure for operating Gas Chromatography
MAKE: Shimadzu, MODEL : GC-2010.
3.0 RESPONSIBILITY:
3.1. Officer-QC
3.2. Executive-QC
4.0. ACCOUNTABILITY:
Manager-Quality Control
5.0. DEFINITION:
Nil
6.0. PROCEDURE:
6.1. SYSTEM SUITABILITY
Run for every analysis in replicate 4 at the beginning and 2 at the end. Calculate RSD for retention time.
6.2. COLUMN AND INJECTION PORT
6.2.1. Inject 1ml of mixture of methanol and toluene (1:10) at the following conditions.
a. Mobile phase : Nitrogen gas,
b. Injector port temperature : 150°C,
c. Detector temperature : 150°C FID,
d. Column temperature : 120°C.
Inject in replicate and calculate the RSD for retention time.
Limit : NMT 2.0
Incase of abnormality.
a. Change the septum,
b. Clean the injection port
d. Purge the nitrogen gas through column for 2 hours
6.3. FREQUENCY OF CALIBRATION: Once in 3 months
7.0. REFERENCES:
Nil
8.0. ENCLOSURES:
Report format
9.0. ABBREVIATIONS:
Nil
NAME OF THE INSTRUMENT : GAS CHROMATOGRAPHY
INSTRUMENT CODE : xxxx
MODEL : GB-14
MAKE : SHIMADZU
CALIBRATION SOP No. : SOP:QCCB:xxx
INSTRUMENT OPERATION SOP No.: SOP:QCOP:xxx
A. R. No. :
DATE OF CALIBRATION :
NEXT CALIBRATION DUE ON :
CALIBRATION :

REMARKS: